Gray and black bars indicate the time windows during which pupil responses to tone and to Go (behavioral responses) were quantified, respectively. Gray and black arrows indicate tone and stimulus onsets, respectively. Right: Mean pupil diameter (± s.e.m.) separated by trial types. Left: LC spike raster separated by trial types. ( a) Example LC recording with pupil tracking. The example pupil size is typical for all sessions ( Figure 1-figure supplement 1). Trace is from a brief period of non-performance during a behavioral session and so there are no licks. ( e) Example simultaneously recorded LC activity, S1 V m, and pupil with auditory cue and whisker stimulation onsets indicated. Right: Typical wide waveforms of LC units and an electrolytic lesion (arrow: lesion site) in the LC (white) showing the recording location. Middle: LC unit responses to 12 blue light pulses (200 ms) aligned to individual pulse onset. ( d) Left: Responses of a ChR2-expressing LC unit to opto-tagging (lightning bolts: blue light pulses) and tail pinch. ( c) Expression of ChR2 in a Dbh Ai32 mouse (ChR2-EYFP: green tyrosine hydroxylase TH: red). ( b) Schematic of tetrode recording in LC, whole-cell recording in S1, and pupil tracking during the task. MATLAB® is a product of MathWorks.( a) Task schematic, trial structure, and all trial types of the single-whisker detection task ( Yang et al., 2016). ![]() You will need to supply an appropriate computer with MATLAB® software installed. Resonant Scanner for 60mm cage based microscope w/ SI 5ġ Please note, ScanImage Premium Bundles are not turnkey packages. SI 5 systems 1 - Includes RESSCAN-MOM or RESSCAN-GEN, preconfigured NI-FLEX RIO data acquisition system, and two FEMTO DHPCA-100 fast preamps with power supplies. Resonant Scanner for 60mm cage based microscope w/ ScanImage Premium Resonant Scanner for Sutter MOM w/ ScanImage Premium ScanImage Premium Systems 1 - Includes SIP-SOFT, RESSCAN-MOM or RESSCAN-GEN, preconfigured NI-FLEX RIO data acquisition system, and two FEMTO DHPCA-100 fast preamps with power supplies. Please contact your local distributor or Sutter Instrument for a quotation. Integration with WaveSurfer enables synchronized electrode recording and general data recording.MatLab interface offers user scripting and custom analysis capabilities with a full hardware control API exposed for automating experiments.FPGA based continuous, gap-free data acquisition for real-time image processing and future closed loop data processing.Step and settle volume imaging with piezos or Optotune.Continuous volume imaging with fast piezo support (PI, nPoint).Multi-region of interest mode provides targeted sequential imaging and photostimulation.Full support for simultaneous imaging and photostimulation applications requiring parallel scanner paths. ![]()
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